Chapter 1 Get Started
How to Use This Guide
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C
T
Experiments
12
Notes
Other Reagents
If you use fluorescence-based reagents other than TaqMan
®
reagents, you must design
your experiment using Advanced Setup instead of the Design Wizard (see “Advanced
Setup Workflow” on page 204).
For More
Information
For more information on real-time PCR experiments, PCR options, and reagents, refer to
the Real Time PCR System Reagent Guide.
How to Use This Guide
This guide functions as both a tutorial and as a guide for performing your own
experiments.
Using This Guide
as a Tutorial
Using the example experiment data provided with the 7500 software, you can use this
guide as a tutorial for performing a relative standard curve or comparative C
T
experiment
on a 7500/7500 Fast system. Follow the procedures in the appropriate chapters shown in
the table below:
For more information, see “About the Example Experiments” on page 14.
Chapter
Procedure
Relative
Standard Curve
Comparative
C
T
2 6 Design the experiment using the Design Wizard in the
7500 software.
3 7 Prepare the experiment using the reagents and
volumes calculated by the Design Wizard in Chapter 2
(relative standard curve experiment) or Chapter 6
(comparative C
T
experiment).
4 8 Run the experiment on a 7500/7500 Fast instrument.
5 9 Analyze the results of an example experiment.
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