abi 7500 - Advanced Setup Workflow

Appendix A Alternate Experiment Workflows
Advanced Setup Workflow
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C
T
Experiments
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Notes
Advanced Setup Workflow
When you create an experiment using Advanced Setup in the 7500 software, you can set
up the experiment according to your own design.
1. Double-click (7500 software) or select StartAll Programs
Applied Biosystems7500 Software<software name>
where <software name> is the current version of the 7500 software.
2. In the Home screen, click Advanced Setup.
Note: If you do not see the Advanced Setup icon, click the arrow beneath the
Design Wizard icon to expand the Set Up menu.
3. Complete the setup screens to set up a new experiment:
a. Click Experiment Properties (default), enter the experiment name, then
select the experiment properties.
b. Click Plate Setup:
c. Click Add Biological Group to create biological replicates and assign
replicates to samples for each biological group in the reaction plate.
d. Click Run Method, review the reaction volume and thermal profile, then
edit as needed.
e. Click Reaction Setup, review the components and calculated volumes for
the PCR reactions, then edit as needed.
f. (Optional) Click Materials List, review the list of materials, then order
the materials you need to prepare the reaction plate.
Experiment Type Action
Genotyping Define the SNP assays, then assign them to wells in the
reaction plate.
All other experiments Define the targets, then assign them to wells in the
reaction plate.