abi 7500 - Analyze the Relative Standard Curve Experiment

Chapter 5
75
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C
T
Experiments
Notes
Analyze the Relative Standard
Curve Experiment
This chapter covers:
■ Chapter Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
Section 5.1 Review Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
■ Analyze the Experiment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
■ View the Standard Curve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
■ View the Amplification Plot . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
■ View the Gene Expression Plot and Well Table . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
■ Publish the Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
Section 5.2 Troubleshoot (If Needed) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99
■ View the Analysis Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100
■ View the QC Summary. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103
■ Omit Wells from the Analysis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
■ View the Multicomponent Plot. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
■ View the Raw Data Plot . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109
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