abi 7500 - Page 153

Chapter 6 Design the Comparative C
T
Experiment
Review the Reaction Setup
129
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C
T
Experiments
Notes
4. Review the calculated volumes for the sample dilutions:
Print Reaction Setup Instructions
Print reaction setup instructions for use in Chapter 7, “Prepare the Comparative C
T
Reactions.”
1. Click Print Reaction Setup.
2. In the Print Reaction Setup Instructions dialog box, select:
• Detailed Reaction Setup Instructions
• Include Plate Layout
•Use sample color
3. Click Print to print the reaction setup instructions.
4. Click Next.
Sample
Name
Stock Concentration
(ng/µL)
Sample Volume
(µL)
Diluent Volume
(µL)
Total Volume of Diluted Sample
(µL)
Brain 100.0 66.00 66.00 132.00
Kidney 100.0 66.00 66.00 132.00
Liver 100.0 66.00 66.00 132.00
Universal 100.0 66.00 66.00 132.00
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