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abi 7500
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Chapter 6 Design the Comparative C
T
Experiment
Finish the Design Wizard
135
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C
T
Experiments
Notes
The experiment is saved as is and closed.
Note: For the example experiment, do not perform the run at this time.
Finish the
Design Wizard
1. At the bottom of the 7500 software screen, click Finish Designing Experiment.
2. In the Review Plate Layout for Experiment window, review the plate layout. Make
sure there are:
96 Unknown wells
0 Negative Control wells
0 Empty wells
Note: If the plate layout is incorrect, click Return to the Wizard, then check your
entered values.
3. Click Save Experiment.
2
3

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