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Chapter 9 Analyze the Comparative C

Experiment

View the Multicomponent Plot

183

Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve

and Comparative C

Experiments

Notes

View the

Multicomponent

Plot

1. In the navigation pane, select Analysis Multicomponent Plot.

Note: If no data are displayed, click Analyze.

2. Display the unknown wells one at a time in the Multicomponent Plot screen:

a. Click the View Plate Layout tab.

Select one well in the plate layout; the Multicomponent Plot shows the well data.

Note: If you select multiple wells, the Multicomponent Plot screen displays the

data for all selected wells simultaneously.

3. In the Plot Color drop-down list, select Dye.

4. Click (Show a legend for the plot).

Note: This is a toggle button. When the legend is displayed, the button changes to

Hide the plot legend.

5. View the FAM dye signal. In the example experiment, the FAM dye signal increases

throughout the PCR process, which indicates normal amplification.

6. View the ROX dye signal. In the example experiment, the ROX dye signal is

constant throughout the PCR process, which indicates typical data.