Chapter 9 Analyze the Comparative C
T
Experiment
View the Raw Data Plot
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C
T
Experiments
186
Notes
4.
Drag the Show Cycle slider from cycle 1 to cycle 40. In the example experiment, a
stable increase occurs in signal from filter 1, which corresponds to the FAM
™
dye filter.
The filters are:
Analysis
Guidelines
When you analyze your own comparative C
T
experiment, look for the following in each
filter:
• Characteristic signal growth
• No abrupt changes or dips
For More
Information
For more information on the Raw Data Plot screen, open the 7500 Software Help by
clicking or pressing F1.
3
4
Filter
123 45
Dye
•FAM
™
dye
• SYBR
®
Green
dye
•JOE
™
dye
•VIC
®
dye
•TAMRA
™
dye
•NED
™
dye
•Cy3
®
dye
•ROX
™
dye
• Texas Red
®
dye
Cy5
®
dye
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