abi 7500 - Page 222

Chapter 9 Analyze the Comparative C
T
Experiment
Analyze the Study
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C
T
Experiments
198
Notes
4. Click < at the top left of the Replicate Results Data group. The figure below shows
the replicate results and well results tables.
Column Description
Omit For all technical replicates that evaluate the associated sample/target combination:
•A check mark (✓) indicates that all replicates have been removed from the analysis.
•A hyphen (–) indicates that one or more replicates have been removed from the analysis.
Sample The sample associated with the data displayed in the row.
Target The target assay associated with the data displayed in the row.
C
T
Mean The arithmetic average of the technical replicate C
T
values.
∆C
T
Mean The arithmetic average of the technical replicate C
T
values for the sample replicate group.
∆C
T
SE The sample standard deviation of the sample replicate group level C
T
values.
∆∆C
T
The calculated ∆∆C
T
value for the replicate group associated with the test sample.
RQ The calculated relative level of gene expression for the replicate group associated with the test sample.
RQ Min The minimum relative level of gene expression in the test samples calculated using the confidence level set in
the Analysis Settings dialog box.
Note: The minimum includes the variability associated with the endogenous control and targets in only the
test samples.
RQ Max The maximum relative level of gene expression in the test samples calculated using the confidence level set
in the Analysis Settings dialog box.
Note: The maximum includes the variability associated with the endogenous control and targets in only the
test samples.
Flag The number of QC flags the well generated as listed in the ▲ symbol.