Chapter 9 Analyze the Comparative C
T
Experiment
Analyze the Study
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C
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Experiments
200
Notes
View the Amplification Plot
The Amplification Plot displays post-run amplification of the samples of the experiments
added to the study. The plot is identical to the plot of the same name described in the
experiment-level analysis with the exception of the Experiment Data table, which allows
you to select the experiment data displayed by the plot. For more information, see “View
the Amplification Plot” on page 166.
Note: To display a subset of the study data in the Amplification Plot, select one or more
rows in the Experiment Data tab or the Well Table tab, then select Hide unselected data
from plot to display data only from the selected rows.
Omit Wells using the Well Table
You can use the Well Table to omit individual wells from the analysis. To omit a well:
1. In the Experiment Data group, select the experiment that contains the well of
interest.
2. In the View Well Table tab, select the check box in the Omit column for the well of
interest.
IMPORTANT! You cannot omit all technical replicates that belong to a reference sample,
belong to a reference biological group, or serve as the endogenous control.
View the Multicomponent Plot
The Multicomponent Plot displays complete spectral contribution of each dye over the
duration of the PCR run in a selected well of any experiment added to the study. The plot
is identical to the plot of the same name described in the experiment-level analysis with
the exception of the Experiment Data table, which allows you to select the experiment
data displayed by the plot. For more information, see “View the Multicomponent Plot” on
page 182.
Note: To display a subset of the study data in the Multicomponent Plot, select one or
more rows in the Experiment Data tab or the Well Table tab, then select Hide unselected
data from plot to display data only from the selected rows.
Note: You can use the View Well Table tab of the Multicomponent Plot to omit
individual wells from the analysis. The procedure for omitting wells is identical to that
for the Amplification Plot. See “Omit Wells using the Well Table” above.
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