abi 7500 - Page 235

Appendix A Alternate Experiment Workflows
Export/Import Workflow
211
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C
T
Experiments
Notes
c. In the Home screen, click Advanced Setup.
Note: If you do not see the Advanced Setup icon, click the arrow below the
Design Wizard icon to expand the Set Up menu.
d. Create a new experiment or open an existing experiment.
e. Select FileImport.
f. Click Browse, locate and select the text file (*.txt), then click Select.
g. Click Start Import. The setup data from the exported text file is imported into
the open experiment.
Note: If your experiment already contains reaction plate setup information, the
software prompts you to replace the reaction plate setup with the data from the
text file. Click Ye s to replace the reaction plate setup.
2. Use Advanced Setup to finish setting up your experiment (see page 204).
3. Prepare the PCR reactions:
4. Run the experiment:
IMPORTANT! While the 7500/7500 Fast instrument is performing a run, do not
create experiments, perform maintenance, or allow the computer to run antivirus
software or to enter hibernation. Performing such activities while the instrument is
running an experiment will cause gaps in data collection.
a. Load the reaction plate into the instrument.
b. Start the run.
c. (Optional) Monitor the run.
d. Unload the reaction plate from the instrument.
Experiment Type Prepare the…
Relative standard curve a. Template.
b. Sample dilutions.
c. Standard dilution series.
d. Reaction mix.
e. Reaction plate.
Standard curve
Comparative C
T
a. Template.
b. Sample dilutions.
c. Reaction mix.
d. Reaction plate.
Genotyping
Presence/absence