Glossary
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C
T
Experiments
222
QuickStart
A feature in 7500/7500 Fast systems that allows you to run an experiment without entering
plate setup information.
R
2
value
Regression coefficient calculated from the regression line in the standard curve. The R
2
value indicates the closeness of fit between the standard curve regression line and the
individual C
T
data points from the standard reactions. A value of 1.00 indicates a perfect fit
between the regression line and the data points.
ramp
The rate at which the temperature changes during the instrument run. Except for the melt
curve step, the ramp is defined as a percentage. For the melt curve step, the ramp is defined
as a temperature increment. In the graphical view of the thermal profile, the ramp is
indicated by a diagonal line.
ramp speed
Speed at which the temperature ramp occurs during the instrument run. Available ramp
speeds include fast and standard.
• For optimal results using the fast ramp speed, Applied Biosystems recommends using
TaqMan
®
Fast reagents in your PCR reactions.
• For optimal results using the standard ramp speed, Applied Biosystems recommends
using standard reagents in your PCR reactions.
IMPORTANT!
TaqMan Fast reagents are not supported for genotyping or
presence/absence experiments.
raw data plot
A plot of raw fluorescence (not normalized) for each optical filter.
reaction mix
A solution that contains all components to run the PCR reaction, except for the template
(sample, standard, or control).
reagents
The PCR reaction components you are using to amplify the target and to detect
amplification. Types of reagents used on the 7500/7500 Fast systems:
• TaqMan
®
reagents
•SYBR
®
Green reagents
• Other reagents
real-time PCR
Process of collecting fluorescence data during PCR. Data from the real-time PCR are used
to calculate results for quantitation experiments or to troubleshoot results for genotyping or
presence/absence experiments.
reference sample
In relative standard curve and comparative C
T
(
∆∆
C
T
) experiments, the sample used as the
basis for relative quantitation results. Also called the calibrator.
refSNP ID
Identifies the reference SNP (refSNP) cluster ID. Generated by the Single Nucleotide
Polymorphism Database of Nucleotide Sequence Variation (dbSNP) at the National Center
for Biotechnology Information (NCBI). The refSNP ID can be used to search the Applied
Biosystems Store for an Applied Biosystems SNP Genotyping Assay. Also called an rs
number.
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