abi 7500 - Page 254

Index
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C
T
Experiments
230
∆C
T
Mean 164, 198
∆C
T
SD 164
∆C
T
SE 198
∆∆C
T
198
∆∆C
T
SD 164
Flag 198
Normalized Qty Mean 95
Normalized Qty Std Err 95
Omit 95
, 164, 198
RQ 95
, 164, 198
RQ Max 95
, 164, 198
RQ Min 95
, 164, 198
Sample 95
, 164, 198
Target 95
, 164, 198
comparative CT example experiment
analyze 156
description 15
design 112
, 189, 195
name 114
prepare 138
workflow 17
, 18
comparative CT experiments
about 8
compared to relative standard curve experiments 9
comparative CT study
analyze 195
design 189
, 190, 192
properties 190
workflow 18
, 156
comparative method of calculation 188
Compare Analysis Settings screen 202
compare, analysis settings 202
consumables
Also see materials required 4
supported 4
conventions
safety xii
conventions used in this guide viii
create new
experiment 21
, 113
study 189
C
T
Mean column 95, 164, 198
CTFAIL flag 104
, 180
Custom assays 42
, 130
customer feedback, on Applied Biosystems
documents xi
D
DANGER, description xii
data
about data collection 2
example experiment 12
, 78, 158
example study 188
publish 174
∆C
T
Mean column 164, 198
∆C
T
SD column 164
∆C
T
SE column 198
∆∆C
T
column 198
∆∆C
T
experiment. See comparative C
T
experiment
∆∆C
T
SD column 164
Define Replicates screen 192
design experiment
create new 21
, 113
define experiment properties 22
, 114
define methods and materials 24
, 116
finish Design Wizard 46
, 134
for more information 23
, 26, 28, 31, 33, 34, 36, 42,
46, 48, 115, 118, 120, 123, 124, 126, 130,
134, 136
guidelines 23
, 25, 28, 30, 32, 34, 36, 42, 46, 48,
115, 117, 120, 122, 124, 126, 130, 134, 136
order materials 43
, 131
review reaction setup 36
, 127
set up relative quantitation settings 34
, 124
set up run method 35
, 125
set up samples 31
, 121
set up standards 29
set up targets 26
, 119
workflow 20
, 112
design study
create new 189
define replicates 192
define study properties 190
for more information 192
guidelines 191
, 194
Design Wizard
Experiment Properties screen 22
, 114
finish 46
, 134
Materials List screen 43
, 131
Methods & Materials screen 24
, 116
Reaction Setup screen 36
, 127
Relative Quantitation Settings screen 34
, 124
Run Method screen 35
, 125
Samples screen 31
, 121
Standards screen 29
Targets screen 26
, 119
deviation, standard 91
, 172
documentation, related ix
E
electrical safety xx
electromagnetic compatibility standards.
See EMC standards
EMC standards xxii
endogenous control
component of experiment 7
, 8
Relative Quantitation Settings screen 34
, 124
selecting 28
, 120
setting multiple endogenous controls 101, 177
ergonomics, safety xxi
example experiment, run 66
, 152
Experiment Properties screen 22
, 114