Chapter 1 Get Started
About the Example Experiments
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C
T
Experiments
16
Notes
About the
Example
Experiment Data
In this getting started guide, you use five files:
• In Chapter 2, you create a relative standard curve example experiment that contains
setup data, then you save the file to your computer.
• In Chapter 5, you view results in a relative standard curve example experiment file
that contains run data. The data file for the example experiments is installed with the
7500 software. You can find the data file for the example experiment on your
computer at:
<drive>:\Applied Biosystems\<software name>\experiments\
Relative Standard Curve Example.eds
• In Chapter 6, you create a comparative C
T
example experiment that contains setup
data, then you save the file to your computer.
• In Chapter 9, you view results in a comparative C
T
example experiment file that
contains run data and in a comparative C
T
example study file that contains the data
from multiple comparative C
T
experiments. The data files install with the 7500
software, and are on your computer at:
–<drive>:\Applied Biosystems\<software name>\experiments\
Comparative Ct Example.eds
–<drive>:\Applied Biosystems\<software name>\experiments\
Comparative Ct Study Example.edm
where:
–<
drive
> is the computer hard drive on which the 7500 software is installed.
–<
software name
> is the current version of the 7500 software.
Example Files in the Experiments Folder
The experiments folder of the 7500 software contains several example files that you can
reference when analyzing your own data. The following example files install with the
7500 software:
• Comparative Ct Example.eds
• Comparative Ct Study Example.edm
• Comparative Ct Study (Biological Groups).edm
• Genotyping Example.eds
• Presence Absence Example.eds
• Relative Standard Curve Example.eds
• Standard Curve Example.eds
Note: Be sure to use the Comparative Ct Example.eds, Relative Standard Curve
Example.eds, and Comparative Ct Study Example.edm files when you perform the
procedures in this guide.