abi 7500 - Page 92

Chapter 4 Run the Relative Standard Curve Experiment
Prepare for the Run
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C
T
Experiments
68
Notes
Load the
Reaction Plate
into the
Instrument
PHYSICAL INJURY HAZARD. During operation, the sample
block can be heated to 100 °C. Before performing the following procedure, be sure to
wait until the sample block reaches room temperature.
IMPORTANT! Wear powder-free gloves when you handle the reaction plate .
1. Push the tray door to open it.
2. Load the plate into the plate holder in the instrument. Ensure that the plate is
properly aligned in the holder.
3. Place the reactions in the precision plate holder (PPH). If you use:
• A reaction plate – Place the reaction plate in the PPH with well A1 at the
back-left corner.
• Reaction tube strips – Place the tube strips in the PPH for tube strips.
Note: Fast Reaction 8-tube strips can be used only on the 7500 Fast system.
For the 7500 system, use MicroAmp
®
Optical 8-Tube Strips.
GR2477
7300/7500
barcode on plate
GR2477
7500 system
Load standard plates with
the notched A12 position at
the top-right of the tray.
7500 Fast system
Load Fast plates with the
notched A1 position at the
top-left of the tray.
GR2475
GR2475
7500
keyed corner
A
B
C
D
E
F
G
H
123456789101112
A
1
GR2475
GR2475
7500
keyed corner
A
B
C
D
E
F
G
H
123456789101112
A
1