Chapter 4 Testing samples
54
OsmoTECH XT Single-Sample Micro-Osmometer User Guide
2. With your thumb on the plunger top and fingers
grasping the barrel, depress the plunger; then insert
the tip into the liquid sample at least 1/4" (6 mm)
below the surface.
Gently release the plunger to load a 20-μL sample.
3. Look at the sample you have just drawn. If there are
voids or bubbles in the sample, discard it and load
another sample that does not contain voids.
4. Remove any sample on the outside of the tip using a
clean, lint-free, non-ionic paper. Quickly swipe the end
of the sampler tip to remove any excess sample
protruding beyond the tip.
NOTE: Do not remove any of the sample below the
acceptable meniscus line (proper sample level
shown below).
Meniscus
Acceptable
Before wiping
Unacceptable
Sample
Plastic disposable tip
Teflon plunger tip
5. Holding the sampler by the barrel, carefully insert the
tip into the sample port; then rest the sampler body in
the operating cradle.
6. Grasp the operating cradle and push it slowly forward
until you feel a positive stop. The test starts when the
cradle reaches the forward position.
When the test begins, the progress bar on the screen
begins to fill. The status bar at the top of the display
is yellow, and the status is TESTING.
7. Wait while the OsmoTECH XT performs the test.
The OsmoTECH XT first cools the sample. After a
moment, you will hear the solenoid impact one or two
times during the last stages of testing. This is normal
operation.
When the test completes, the resulting osmolality
displays in the middle of the screen. The software
provides the instruction to remove the sampler and
clean the chamber.
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