12 Purification Solution - Developer's Guide
2
Preparing Default Purification Methods
General Method Settings
Prep Pump (G7161A)
Prep Pump (G7161B)
Advanced Channel A Set Compressibility to 46 (Water)
Advanced Channel B Set Compressibility to 115 (Acetonitrile)
Solvents A
Select Aqueous and type the description text 
Water
.
B
Mark the check box, enter 
2 %
, select Acetonitrile, and type description text 
Acetonitrile
.
Pressure Limits Set Max limit: find the pressure limit of the columns used (for 
example, 21.2 mm guard system seals finger-tight up to 135 bar; 
21.2 mm prep column without the guard up to 350 bar).
Advanced In Compressibility, keep the check box Use Solvent Types 
marked
Solvents A
Select solvent line 1 or 2, select Aqueous and type the 
description text 
Water
.
B
Mark the check box, select solvent line 1 or 2, enter 
2 %
, select Acetonitrile, and type description text 
Acetonitrile
.
Pressure Limits Set Max limit: find the pressure limit of the columns used (for 
example, 21.2 mm guard system seals finger-tight up to 135 bar; 
21.2 mm prep column without the guard up to 350 bar).
Advanced In Compressibility, keep the check box Use Solvent Types 
marked