54 Agilent 2200 TapeStation System - User Manual
4 Using the 2200 TapeStation System
DNA Sample Preparation
Mixing recommendations
• 2200 TapeStation instruments are supplied with an optional IKA MS3 vortexer which
includes a 96-well plate adaptor suitable for both 96-well PCR plates and 8-way strips.
• This vortexer is recommended for use with all ScreenTape assays with the exception of
the P200 ScreenTape assay which should be manually vortexed.
• It is recommended that all current 2200 TapeStation system users purchase this
instrument direct from IKA for use with these assays. Please quote part number
4674100 when ordering.
• Agilent Technologies will not sell these parts separately.
• If an IKA MS3 vortexer is not available, please ensure thorough manual vortex mixing -
10 seconds on maximum speed.
For successful loading, the sample solution must be placed at the bottom of the tube or
well without any air-bubbles; centrifugation of samples after preparation is essential. The
2200 TapeStation instrument will load a sample from a minimum of 3 µL onto the
ScreenTape device.
For best sizing precision and accuracy, the user should run the appropriate ladder with the
samples.
If 16 samples need to be analyzed in parallel, you may choose to insert an electronic ladder
in the TapeStation Analysis Software.
Sizing results may not be as accurate using an electronic ladder.
Electronic ladder functionality is not available for D5000, High Sensitivity D5000, or
Genomic DNA assays.
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