Agilent 2200 TapeStation System - User Manual 65
Using the 2200 TapeStation System
4
RNA Sample Preparation
Sample Preparation High Sensitivity RNA ScreenTape Assay
Prepare TapeStation System HSRNA
1 Launch the 2200 TapeStation Controller Software.
2 Load High Sensitivity RNA ScreenTape device and loading tips into the
2200 TapeStation instrument.
3 Select RNA Protocol (Eukaryotic RNA or Prokaryotic RNA)
Sample Preparation High Sensitivity RNA ScreenTape Assay
1 Allow reagents to equilibrate at room temperature for 30 min
2 Vor tex mix before use
3 Thaw total RNA samples on ice
4 Prepare diluted Ladder solution, by adding 10 µL RNase free water to
the High Sensitivity RNA Ladder vial and mixing thoroughly.
5 If running ladder, prepare by mixing 1 µL High Sensitivity RNA Sample
Buffer ( ) with 2 µL RNA ladder ( )
6 Prepare sample by mixing 1 µL High Sensitivity RNA Sample Buffer ( )
with 2 µL RNA sample
7 Spin down, then vortex using IKA vortexer and adaptor at 2000 rpm
for 1 min.
Parts required p/n Description
5067-5579 High Sensitivity RNA ScreenTape
Parts required p/n Description
5067-5580 High Sensitivity RNA ScreenTape Sample Buffer
5067-5581 High Sensitivity RNA ScreenTape Ladder
For best results, use the reverse pipetting technique.
Freeze any unused Ladder at -20 °C (-4 °F).
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