Agilent 2200 TapeStation System - User Manual 57
Using the 2200 TapeStation System
4
DNA Sample Preparation
Sample Preparation D5000 ScreenTape Assay
Prepare TapeStation System D5000
1 Launch the 2200 TapeStation Controller Software.
2 Load single D5000 ScreenTape device and loading tips into the 2200
TapeStation instrument
Sample Preparation D5000 ScreenTape Assay
1 Allow reagents to equilibrate at room temperature for 30 min
2 Vor tex mix before use
3 Prepare ladder by mixing 10 µL D5000 Sample Buffer ( ) with 1 µL
D5000 Ladder ( ) in a tube strip.
4 Prepare sample by mixing 10 µL D5000 Sample Buffer ( ) with 1 µL
DNA sample in the same tube strip.
5 Spin down, then vortex using IKA vortexer and adaptor at 2000 rpm
for 1 min.
6 Spin down to position the sample at the bottom of the tube.
Parts required p/n Description
5067-5588 D5000 ScreenTape
Parts required p/n Description
5067-5589 D5000 Reagents
Use a fresh ladder for each run. No electronic ladder is available for the D5000 assay.
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