Agilent Technologies 2200 - Sample Preparation Genomic DNA Screentape Assay

Agilent 2200 TapeStation System - User Manual 59
Using the 2200 TapeStation System
4
DNA Sample Preparation
Sample Preparation Genomic DNA ScreenTape Assay
Prepare TapeStation System gDNA
1 Launch the 2200 TapeStation Controller Software.
2 Load Genomic DNA ScreenTape device and loading tips into the 2200
TapeStation instrument.
Sample Preparation Genomic DNA ScreenTape Assay
1 Allow reagents to equilibrate at room temperature for 30 min
2 Vortex mix before use.
3 Prepare ladder by mixing 10 µL Genomic DNA Sample Buffer ( ) with
1 µL Genomic DNA Ladder ( )
4 Prepare sample by mixing 10 µL Genomic DNA Sample Buffer ( ) with
1 µL genomic DNA sample (10 – 100 ng/µL).
5 Spin down, then vortex using IKA vortexer and adaptor at 2000 rpm for
1min.
6 Spin down to position the sample at the bottom of the tube.
Parts required p/n Description
5067-5365 Genomic DNA ScreenTape
Parts required p/n Description
5067-5366 Genomic DNA Reagents
NOTE
Use a fresh ladder for each run. No electronic ladder is available for the Genomic DNA
assay.