Agilent 2200 TapeStation System - User Manual 67
Using the 2200 TapeStation System
4
Protein Sample Preparation
Protein Sample Preparation
• When pipetting sample buffer, ensure that excess buffer droplets are removed from the
tip before transfer to the sample tubes. Care must be taken due to viscosity of Sample
Buffers.
• When pipetting small volumes ensure that no sample remains within the tip.
• When adding sample buffer to sample, please ensure that they are mixed correctly by
following assay instructions.
• Improper mixing can lead to quantification errors.
• Once mixed briefly centrifuge to collect the contents at the base of tubes.
• For best results, ensure that all reagents are allowed to equilibrate to room temperature
for 30 minutes prior to use.
Improper mixing can lead to quantification errors.
For successful loading, the sample solution must be placed at the bottom of the tube or
well without any air-bubbles; centrifugation of samples after preparation is essential. The
2200 TapeStation instrument will load a sample from a minimum of 3 µL onto the
ScreenTape device.
For best sizing precision and accuracy, the user should run the appropriate ladder with the
samples.
If 16 samples need to be analyzed in parallel, you may choose to insert an electronic ladder
in the TapeStation Analysis Software.
Sizing results may not be as accurate using an electronic ladder.
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