Agilent 2200 TapeStation System - User Manual 61
Using the 2200 TapeStation System
4
RNA Sample Preparation
RNA Sample Preparation
Sample degradation
➔ Ensure all working areas, reagents and plastic ware are RNase free.
➔ Handle RNA samples with care.
➔ Wear gloves at all times.
➔ Thaw RNA samples on ice.
➔ Vortex and centrifuge all samples before use.
➔ Store RNA samples on ice throughout the ScreenTape analysis procedure.
• It is important to place the samples on ice directly after the denaturation step as this
aids complete and stable denaturation of the RNA.
• To ensure optimal performance of the RNA ScreenTape assays, samples should be
analyzed using the 2200 TapeStation instrument within 3 h of the denaturation step.
Beyond 3 h denatured samples should be stored on ice or in a suitable freezable sample
block.
• For best results, ensure that all reagents are allowed to equilibrate to room temperature
prior to use.
• When pipetting Sample Buffer, ensure that excess buffer droplets are removed from the
tip before transfer to the sample tubes.
Care must be taken due to the viscosity of Sample Buffers.
• When pipetting small volumes ensure that no sample remains within the tip.
• When adding Sample Buffer to sample, please ensure that they are mixed correctly. To
achieve this, gently mix several times with additional pipetting, then cap the tubes,
vortex mix using IKA vortexer and adaptor at 2000 rpm for 1 min.
• Briefly centrifuge to collect the contents at the base of the tubes.
• Improper mixing can lead to quantification errors.
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