Agilent 2200 TapeStation System - User Manual 53
Using the 2200 TapeStation System
4
DNA Sample Preparation
DNA Sample Preparation
• When pipetting sample buffer, ensure that excess buffer droplets are removed from the
tip before transfer to the sample tubes. Care must be taken due to viscosity of Sample
Buffers.
• When pipetting small volumes ensure that no sample remains within the tip.
• When adding sample buffer to sample, please ensure that they are mixed correctly by
following assay instructions.
• Improper mixing can lead to quantification errors.
• Once mixed briefly centrifuge to collect the contents at the base of tubes.
• For best results, ensure that all reagents are allowed to equilibrate to room temperature
for 30 minutes prior to use.
When using 96-well plates, the use of a 96-well plate vortex adaptor is advised to ensure
correct sample mixing. Improper mixing can lead to quantification errors.
As with samples in 8-way strips, briefly centrifuge after vortexing to collect the contents at
the base of the tubes before placing into the 2200 TapeStation instrument.
D5000 and High Sensitivity D5000 assays are limited to runs using a single ScreenTape
device, and cannot be run from 96-well plates.
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