Agilent 2200 TapeStation System - User Manual 63
Using the 2200 TapeStation System
4
RNA Sample Preparation
Sample Preparation RNA ScreenTape Assay
Prepare TapeStation System RNA
1 Launch the 2200 TapeStation Controller Software.
2 Load RNA ScreenTape device and loading tips into the 2200
TapeStation instrument.
3 Select RNA Protocol (Eukaryotic RNA or Prokaryotic RNA)
Sample Preparation RNA ScreenTape Assay
1 Allow reagents to equilibrate at room temperature for 30 min
2 Vor tex mix before use
3 Thaw total RNA samples on ice
4 If running ladder, prepare by mixing 5 µL RNA Sample Buffer ( ) with
1 µL RNA Ladder ( ).
5 Prepare sample by mixing 5 µL RNA Sample Buffer ( ) with 1 µL RNA
sample.
6 Spin down, then vortex using IKA vortexer and adaptor at 2000 rpm
for 1 min.
Parts required p/n Description
5067-5576 RNA ScreenTape
Parts required p/n Description
5067-5577 RNA ScreenTape Sample Buffer
5067-5578 RNA ScreenTape Ladder
For best results, use the reverse pipetting technique.
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