5 Maintenance
Ion Injector
Agilent 6475 Triple Quad LC/MS System User Guide 81
Cleaning the ion injector
Perform this procedure when you observe decreased sensitivity and decreased
signal stability.
Tools needed:
• Clean powder-free nitrile gloves
• LC/MS-grade isopropanol
• Alconox powdered precision cleaner
• Deionized (18 MΩ/cm) water
• 100-mL polypropylene graduated cylinder
• 10-mL polypropylene graduated cylinder
• Two 1-mL pipette tip
To clean the ion injector,
1 Put on clean powder-free nitrile gloves.
2 Dissolve 1 gram of Alconox Powdered Precision Cleaner in 100 mL of
deionized (18 MΩ/cm) water. This concentration is the recommended
concentration for both manual or ultrasonic cleaning.
3 If you use a glass graduated cylinder, cover the end of the ion injector with a
1-mL pipette tip to protect the metalized plating.
4 Trim the pipette tip to approximately 4 cm so that the ion injector can be
immersed in the cleaning solution.
5 Place the ion injector upright in a graduated cylinder and fill with Alconox
Powdered Precision Cleaner solution.
6 Put the Ultrasonic cleaner under a fume hood. Sonication should be done
under a fume hood.
7 Sonicate the graduated cylinder that contains the ion injector in an ultrasonic
cleaner for 10 to 15 minutes. Do not clean longer than 15 minutes.
Rinse the ion injector and graduated cylinder several times with deionized water.
8 Fill the graduated cylinder with deionized water and sonicate for 10 to 15
minutes.
9 Remove the ion injector from the graduated cylinder and remove the pipette
tips (if any were used).
10 Blow out excess water from the ion injector bore with nitrogen.
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