3Tuning
About Tuning
Agilent 6475 Triple Quad LC/MS System User Guide 28
About Tuning
Autotune and Checktune
A Checktune can be used to determine if the tuning mix ion masses are properly
assigned and if the response or sensitivity of these ions is within expectations. In
other words, a Checktune verifies peak width and mass axis to make sure they
are correct before you start your acquisition. This check tune takes
approximately 3 minutes to run in each polarity. See “Calibrating the LC/TQ
(Checktune)” on page 30.
Autotune only needs to run after preventative maintenance or if you find a
problem with Checktune. You only mark Tune from factory default if regular
Autotune fails, or if the tune file was corrupted. An Autotune can take
approximately 15 to 20 minutes for each polarity. Everything is automatic since
the tuning mix is delivered by the calibrant delivery system, which is switched on
automatically during the tune. See “Calibrating the LC/TQ (Autotune)” on
page 31.
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