12 Agilent 1100 Series Fraction Collectors User’s Guide
1 Configuration and Operation of the Fraction Collector
Configuration of the Fraction Collector
Fraction Delay Volumes
The Fraction Delay Volume specifies the volume between the detector cell and
fraction collector needle tip. This volume has to determined by a delay
calibration. See “Delay volumes and delay calibration” on page 15 for a
detailed description on delay volumes and delay calibration.
Collection Order
The Collection Order describes the way of capillary movement during fraction
collection. Four different settings are possible:
• row-by-row
• column-by-column
• shortest way by row
• shortest way by column
Shortest way by row/column is recommend, if Continuous flow is selected as
Collection mode.
Collection Mode
The Collection Mode defines the triggering of the diverter valve. For Discrete
fractions the diverter valve switches to waste while the needle moves to the
next test tube, vial or well. For Continuous flow the diverter valve is not
switching during needle movements, except for long movements in the
row-by-row or column-by-column mode. In addition the needle cannot move
Into location during continuous flow operation. Continuous flow is only available
for well plates.
Needle Movement
Into location In the into location mode the needle tip moves into the well to
the specified Depth (in mm). This option is only available for the analytical
scale fraction collector in the Discrete fractions mode.
Above location In the above location mode the needle tip stays at the
specified Distance (in mm) above the well during fraction collection.
Contact Control In this mode the needle tip moves down to the well bottom
until is reaches the specified Distance (in mm) between needle tip and the
vial/well bottom. This ensures that the forming droplet is in contact to the
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