Agilent 1100 Series Fraction Collectors User’s Guide 13
Configuration and Operation of the Fraction Collector 1
Configuration of the Fraction Collector
vial/well bottom. During the continuing filling process the needle tip moves
upwards while staying in contact with the surface of the collected liquid. This
option is recommend for low flow rates and small fraction volumes to avoid air
bubbles and accomplish an accurate fraction volume. It is only available for
the analytical scale fraction collector.
Well-Plates
In the Well-Plates section the type of well plates used in a well plate tray can be
configured. The used well plate type can be chosen from the Plate type
dropdown menu. More detailed information about all pre-configured well
plates can be found in the Instrument menu. Only one type of well plate can be
used on the well plate tray.
The checkbox Treat as 4 x 96 is available only for 384 well plates and allows to
split the 384 well plate virtually into 4 separate 96-well plates. This only
changes the collection order. The numbering of the wells remains as indicated
on the plate. The four virtual 96 well plates start at locations A1, A13, I1 and
I13, respectively.
The filling order of each quarter is as specified in the Collection Order section.
When the 384 well plate is split into four equal quarters the order of the four
plates is the same as displayed in the Installed Plates section.
In the Reserved Locations, you have the possibility to specify locations that will
not be used for Fraction Collection (see Table 1).
Tabl e 1 Syntax for the definition of Reserved Locations
Location Syntax Description
Rows A Row A can’t be used
A,B Rows A and B can’t be used
A-D A, B, C and D can’t be used
A-D,F Rows A, B, C, D and F can’t be used
Columns 1 Column 1 can’t be used
1,2 Columns 1, and2 can’t be used
1-4 Columns 1, 2, 3 and 4 can’t be used
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