3Using the Module
Recommendations for Bio-Compatible and Bio-Inert System
1260 Infinity II Flexible Pump User Manual 56
Flushing Procedure
This procedure should be used when salt-containing mobile phases are used.
It has to be performed regularly, at least once a week, or prior a long standby
or off time, to remove salt deposits from the flow path and surfaces in contact
with the solvents. How to prepare the system for shutdown see “Shut Down
the System” on page 43.
The procedure is mandatory for switching from salt-containing mobile phase
to reversed phase applications (or any applications running with high
organics), where the precipitation of salt can occur.
• Flush the column with recommended storage solvent, be sure that this
solvent is compatible with current mobile phase and cannot cause
precipitation.
• Replace the column with a union, replace the salt-containing solvent bottle
with a new bottle of HPLC-grade water at room temperature.
• Clean the bottle head assembly using lint-free wipes to minimize carryover of
remaining salt solution into the new water bottle.
• Autosampler: perform at least 15 min purge with water to remove salt
residues from all lines, both needle wash and seat backflush for Multiwash
option, visually control needle/seat/washport for salt residues, if necessary
manually clean needle/seat/washport.
• Purge each pump channel that has pumped buffer separately, for at least
10 min at 5 mL/min.
• Flush the entire system flow path with water for at least 10 min at 2 mL/min.
During this step, switch the injection valve and the column selection valve (if
installed) position every 1 min, and repeat this until every position has been
selected for at least 5 times.
• Replace water with fresh solvent bottles to minimize salt carryover.
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