1260 Infinity II Flexible Pump User Manual 55
3Using the Module
Recommendations for Bio-Compatible and Bio-Inert System
Recommendations for Bio-Compatible and
Bio-Inert System
• Make sure all supplies (fittings, capillaries, inline filters, columns, etc.) are
bio-inert / bio-compatible.
• Be aware that some columns for bio-related applications have a stainless
steel case and can introduce iron and other metal ions in the flow path.
This may lead to adsorption of susceptible samples like phosphorylated
nucleotides. In this case, use PEEK-lined columns.
• After using the system with high salt concentrations, flush it extensively with
water to prevent blockages caused by salt crystals.
• Reliable operation of 1290 pumps during analysis cannot be guaranteed if
pressure falls below 20 bar. For optimal results, pressure should be at least
50 bar continuously. Therefore, when using columns that create low
backpressure (<50 bar, such as SEC columns with 1290 LC systems), install a
restriction capillary between the pump and the sampler, to achieve at least
50 bar.
• Perform daily flush of the Multisampler with water if the Multiwash option is
installed (see Best Practices for Using an Agilent LC System Technical Note)
Agilent Bio-inert and Bio LC systems should not be subject to passivation or
similar procedures
This can cause irreversible damage to the system's internal surfaces
 Do not perform passivation or similar procedures on bio-inert and
bio-compatible systems.
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