1260 Infinity II Flexible Pump User Manual 69
3Using the Module
Solvent Recommendation for Agilent 1290 Infinity and 1290 Infinity II and 1260
Infinity II Flexible Pumps
Handling of Acetonitrile
Acetonitrile is a solvent that is frequently used in reversed-phase
chromatography. Despite of its common use, it can be a source of issues if not
handled correctly.
As Acetonitrile ages, some residue can get stuck on internal pump surfaces
which can cause issues with valve performance and therefore affect retention
time precision.
When using acetonitrile:
• Use high-quality solvents from renowned suppliers.
• Use fresh solvents and filter them.
• Minimize exposure to light and air/oxygen.
• Choose a bottle size which fits to your application and usage.
• Acids accelerate solvent aging. If possible avoid such additives or refresh
solvents more frequently.
• Pure acetonitrile ages faster. If your application allows, add about 5 % water
and adjust gradient compositions.
• Do not leave acetonitrile in unused systems to avoid aging. If not in use, flush
all solvent lines with a mixture of water and 10 % isopropanol.
• In case of blocked valves, flush the system with hot water. Knock at valves,
flush them (see “Release a Stuck Inlet Valve” on page 174) or ultrasonicate
them, for example in methanol.
Handling of Acids
Acids can corrode stainless steel and other materials in the flow path of LC
systems. For stainless steel, the minimum pH is 2.3 for corrosive acids and pH 1
for non-corrosive acids.
Please note that for non-volatile acids like phosphoric acid or perchloric acid
concentrations increase after evaporation of water. This means that originally
diluted acids can damage parts over time, e.g. because of liquid, which has left
the solvent path through micro leaks. Such systems should be flushed regularly
with pure water and may require shorter maintenance cycles. Using the seal
wash function should be considered for protecting pump heads.
Please also refer to TechNote 01200-90090, which can be downloaded from our
website www.agilent.com.
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