5 How to Optimize the Performance of Your Module
How to Achieve Higher Resolution
1260 Infinity II Flexible Pump User Manual 98
This shows that k and hence resolution can be increased by having shallower
gradients (2 to 5 %/min change is a guideline), higher flow rates and smaller
volume columns. This equation also shows how to speed up an existing gradient
– if the flow is doubled but the gradient time is halved, k
*
remains constant and
the separation looks the same but happens in half the time. Recently published
research has shown how a shorter STM column (at temperatures above 40 °C)
can generate higher peak capacity than a longer STM column by virtue of running
it faster. (Refer to Petersson et al., J.Sep.Sci, 31, 2346-2357, 2008, Maximizing
peak capacity and separation speed in liquid chromatography).
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