Agilent InfinityLab LC Series Diode Array Detectors User Manual 82
5 Optimizing the Detector
Optimization Overview
The upper limit of concentration
Care should be taken in methods where high background absorption of solvents
or modifiers are present. When using the 60 mm cell the detector will measure 6
times the background absorption as in case of the 10 mm cell, which will reduce
the remaining dynamic absorbance range for sample peaks. Furthermore those
UV absorbing modifiers could compromise the sensitivity gain (signal/noise) of
60 mm cell.
The linearity limit of the detector is seen at about 2 AU for both, the 10 mm and
the 60 mm Max-Light Cartridge Flow Cell. Using firmware revision B.06.25 and
below, the 60 mm Max-Light Cartridge Cell linearity limit would be 333 mAU/cm.
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