Agilent InfinityLab LC Series Diode Array Detectors User Manual 90
5 Optimizing the Detector
Optimizing for Sensitivity, Selectivity, Linearity and Dispersion
Figure 25 Gradient Analysis of PTH-Amino Acids (1 pmol each), with and without Reference
Slit Width (G7117B)
The 1290 Infinity DAD (G7117B) has a variable slit at the entrance of the
spectrograph. This is an effective tool to adapt the detector to changing demand
of different analytical problems.
A narrow slit provides spectral resolution for analytes with very fine structures in
the absorbance spectrum. An example of such a spectrum is benzene. The five
main absorbance bands (fingers) are only 2.5 nm wide and just 6 nm apart from
each other.
Time (min)
Grad.: 0.02 m KH PO /ACN from 12% ACN to 45% ACN in 12 min
1pmol each
Reference 380nm
Wavelength
267 nm
Wavelength
267nm
No reference
PTH-ASN
PTH-ARG
PTH-ALA
PTH-PHE
PTH-PRO
24
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