Agilent InfinityLab LC Series Diode Array Detectors User Manual 89
5 Optimizing the Detector
Optimizing for Sensitivity, Selectivity, Linearity and Dispersion
Figure 24 Influence of Bandwidth on Signal and Noise
Because the detector averages absorbance values that are calculated for each
wavelength, using a wide bandwidth does not negatively impact linearity.
The use of a reference wavelength is highly recommended to further reduce
baseline drift and wander induced by room temperature fluctuations or refractive
index changes during a gradient.
An example of the reduction of baseline drifts is shown in Figure 25 on page 90
for PTH-amino acids. Without a reference wavelength, the chromatogram drifts
downwards due to refractive index changes induced by the gradient. This is
almost completely eliminated by using a reference wavelength. With this
technique, PTH-amino acids can be quantified in the low picomole range even in
a gradient analysis.
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