DRAFT
September 25, 2007 1:07 am, 4376782_Maintain_the_System.fm
Chapter 6 Maintain the System
Regular Maintenance
91
Applied Biosystems StepOne
™
and StepOnePlus
™
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
Notes
4. Remove calibration plate 1 from its packaging.
5. Vortex calibration plate 1 for 5 sec.
6. Centrifuge calibration plate 1 for 2 min at less than1500 rpm.
IMPORTANT! The calibration plate must be well mixed and centrifuged.
7. Confirm that the liquid is at the bottom of each well of calibration plate 1. If not,
centrifuge the calibration plate again at a greater rpm and for a longer time.
IMPORTANT! Do not allow the bottom of the calibration plate to become dirty.
Fluids and other contaminants that adhere to the bottom of the plates can
contaminate the sample block(s) and cause an abnormally high background signal.
8. Load calibration plate 1 into the instrument:
a. Open the instrument drawer.
b. Place the calibration plate in the sample block(s) so that the A1 position is at
the back-left corner.
c. Close the instrument drawer carefully.
Correct Incorrect
Liquid is at the
bottom of the well.
• Not centrifuged with enough force,
or
• Not centrifuged for enough time
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