Chapter 1: Introduction 21
Collection Optics
From the flow cell, laser light is routed to the collection optics, which efficiently
gather the signals emitted and scattered from each particle. The BD FACSCanto
collection optics include two detector arrays, which consist of photomultiplier
tubes (PMTs) arranged in one octagon and one trigon (Figure 1-3).
The octagon contains five PMTs and detects light from the 488-nm (blue) laser.
One PMT in the octagon collects side scatter (SSC) signals.
The trigon contains two PMTs and detects light from the 633-nm (red) laser.
Figure 1-3 Octagon and trigon detector arrays
A
C
B
D
F
H
A
C
E
G
B
670LP
780/60
660/20
530/30
488/10
780/60
585/42
735LP
655LP
502LP
735LP
556LP
octagon
blue-laser
signal
trigon
longpass
mirror
bandpass
filter
red-laser
signal
PMT
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