22 BD FACSCanto Flow Cytometer Reference Manual
When light arrives at an array, a longpass mirror (filter) transmits the highest
wavelengths to the first PMT in the series and reflects lower wavelengths to the
next PMT. Similarly, the next PMT’s longpass mirror transmits the next highest
wavelengths and reflect lower wavelengths, and so on around the array. A
bandpass filter (or additional longpass mirror) in front of each PMT further
screens unwanted light.
In addition to the PMT detectors, a photodiode collects the stronger forward
scatter signals. The obscuration bar prevents excess laser light from entering this
diode (Figure 1-1 on page 18).
For additional descriptions of detector arrays, mirrors, and filters, see Optics
System on page 109.
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