Chapter 1: Introduction 23
At installation, the octagon and trigon arrays have the filter and mirror
combinations shown in Table 1-1.
Blank optical holders do not contain optical filters. They are used in the octagon
and trigon to prevent unwanted light from interfering with fluorescence signal.
Table 1-1 Octagon and trigon optical filters
Detector Array
(Laser)
PMT
Position
LP Mirror
BP Filter or
LP Mirror
Intended Dye
Octagon
(488-nm blue laser)
A 735 780/60 PE-Cy7
B 655 670 PerCP-Cy5.5 or
PerCP
C 556 585/42 PE
D 502 530/30 FITC
Eblank
optical holder
488/10 SSC
Fblank
optical holder
blank
optical holder
—
Gblank
optical holder
blank
optical holder
—
H— blank
optical holder
—
Trigon
(633-nm red laser)
A 735 780/60 APC-Cy7
B blank 660/20 APC
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