Binder CB Series - Page 6

CB / CB-UL, CBF / CBF-UL (E7) 01/2022 Page 6/199
19. REFERENCE MEASUREMENTS ...................................................................... 146
19.1 CO
2
reference measuring ............................................................................................................... 146
19.1.1 Measuring CO
2
concentration indirectly via the pH of the cell medium ................................ 146
19.1.2 Measuring CO
2
directly via chemical indicator tubes............................................................ 147
19.1.3 Measuring CO
2
directly with an electronic infrared measuring device ................................. 147
19.2 Temperature reference measurement ............................................................................................ 147
20. AVOIDING MICROBIAL CONTAMINATION...................................................... 148
20.1 Cells and media .............................................................................................................................. 148
20.2 Laboratory conditions / equipment around the incubator ................................................................ 148
20.3 Working and behavior in the lab ..................................................................................................... 148
20.4 Chamber design and equipment of the CO
2
incubator ................................................................... 149
20.5 Handling the CO
2
incubator ............................................................................................................ 150
21. CLEANING, DECONTAMINATION / DISINFECTION, AND STERILIZATION .. 151
21.1 Cleaning .......................................................................................................................................... 151
21.2 Decontamination / chemical disinfection of the chamber ................................................................ 153
21.2.1 Disinfection of the CO
2
sensor .............................................................................................. 154
21.2.2 CBF / CBF-UL: Disinfection of components of the fresh water supply system .................... 154
21.3 Hot-air sterilization .......................................................................................................................... 155
21.3.1 Overview ............................................................................................................................... 155
21.3.2 Preparation for a hot-air sterilization ..................................................................................... 156
21.3.3 Starting the sterilization cycle and running the hot-air sterilization ....................................... 156
21.3.4 Prematurely terminating the sterilization cycle ..................................................................... 158
21.3.4.1 Prematurely terminating the sterilization cycle after less than 4 hours: Ineffective
sterilization ....................................................................................................................
158
21.3.4.2 Prematurely terminating the sterilization cycle after more than 4 hours, i.e., during the
cooling-down phase: successful sterilization ................................................................ 159
21.3.5 Completing the entire sterilization cycle ............................................................................... 160
22. MAINTENANCE AND SERVICE, TROUBLESHOOTING, REPAIR, TESTING . 161
22.1 General information, personnel qualification................................................................................... 161
22.2 CBF / CBF-UL: Replacing components of the humidifying / dehumidifying system ....................... 162
22.2.1 Replacing the silicone tube of the fresh water supply system .............................................. 162
22.2.2 Replacing the pump head of the fresh water supply system ................................................ 162
22.2.3 Replacing the gas fine filter (sterile filter) for ambient air ..................................................... 162
22.3 Replacing the CO
2
und O
2
/N
2
gas inlet fine filters (sterile filters) .................................................... 163
22.4 Replacing the CO
2
sensor ............................................................................................................... 164
22.5 Maintenance intervals, service ........................................................................................................ 165
22.6 Simple troubleshooting .................................................................................................................... 166
22.6.1 General ................................................................................................................................. 166
22.6.2 Heating .................................................................................................................................. 167
22.6.3 Gas cylinder pressure too low .............................................................................................. 168
22.6.4 Gas control ............................................................................................................................ 169
22.6.5 Sterilization ........................................................................................................................... 171
22.6.6 Humidity (CB / CB-UL) .......................................................................................................... 171
22.6.7 Humidity (CBF / CBF-UL) ..................................................................................................... 172
22.6.8 Controller .............................................................................................................................. 172
22.6.9 Open door ............................................................................................................................. 173
22.7 Sending the chamber back to BINDER GmbH ............................................................................... 173
23. DISPOSAL.......................................................................................................... 174
23.1 Disposal of the transport packing .................................................................................................... 174
23.1.1 Outer chamber packing......................................................................................................... 174
23.1.2 Packing inside the chamber and equipment ......................................................................... 174
23.2 Decommissioning ............................................................................................................................ 175
23.3 Disposal of the chamber in the Federal Republic of Germany ....................................................... 175