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GE Biacore X100 User Manual

GE Biacore X100
93 pages
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Biacore
X100
Operating Instructions
Original instructions
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GE Biacore X100 Specifications

General IconGeneral
TypeSurface Plasmon Resonance (SPR) System
ApplicationBiomolecular interaction analysis
Detection MethodSurface Plasmon Resonance (SPR)
Temperature Control4-40°C
SoftwareBiacore X100 Control Software
Power Requirements100-240 V, 50/60 Hz
Sample TypeProteins, nucleic acids, small molecules
Flow Rate Range1-100 μL/min
Data AnalysisAffinity, kinetics, concentration, specificity
Flow SystemMicrofluidic
Dimensions60 cm (W) x 50 cm (D) x 45 cm (H)
ThroughputHigh throughput
Sample CapacityUp to 96 samples

Summary

1 Introduction

About this Manual

Explains the manual's purpose and defines typographical conventions used for clarity and consistency.

Important User Information

Covers essential user guidance, intended use, prerequisites, and safety notices for operating the Biacore X100.

Regulatory Information Overview

Details applicable regulations, standards, and manufacturing information for the Biacore X100 system.

Associated Documentation

Lists available user documentation and guides users on how to access related literature online.

2 Safety Instructions

Safety Precautions

Details general safety measures, personal protection, and handling of hazardous substances for Biacore X100 operation.

Product Labels and Symbols

Describes product labels, their components, and the meaning of various safety and regulatory symbols.

Emergency Procedures

Provides instructions for emergency shutdown, power failure response, and system restart procedures.

Recycling and Disposal Information

Outlines procedures for decontaminating, decommissioning, and recycling the Biacore X100 and its components.

3 System Description

Biacore X100 Instrument Overview

Identifies and describes the main physical parts and components of the Biacore X100 instrument.

Indicators and Switches

Explains the function of status indicators on the front panel and the location of the mains power switch.

Sensor Chip Details

Details the structure and components of the Biacore sensor chip, including its frame and cassette.

Control System Software

Describes the Biacore X100 Control and Evaluation Software, their functions, and integrated support features.

4 Installation

Site Requirements

Details necessary site conditions, space, electrical power, and environmental requirements for installation.

Unpacking, Assembly, and Transport

Provides guidance on safely unpacking, assembling, and transporting the Biacore X100 instrument.

Instrument Connections

Explains how to connect the Biacore X100 to a computer and to the mains power source.

5 Operation

Starting and Initializing the System

Guides users on starting the Biacore X100 from shutdown or standby and initiating software.

Sensor Chip Handling

Provides step-by-step instructions for inserting, docking, and managing sensor chips in the instrument.

Sample and Reagent Management

Covers preparation, loading, and management of samples, reagents, and buffers for experiments.

Running Experiments and Post-Run Procedures

Details the process of starting a run, monitoring its progress, and performing necessary post-run operations.

6 Maintenance

Maintenance Preparations and Schedule

Outlines essential preparations, safety guidelines, and a regular maintenance schedule for the instrument.

Cleaning and Sanitizing Flow System

Provides detailed procedures for cleaning and sanitizing the instrument's flow system and components.

Component Maintenance and Fuses

Covers checks for leaks, system performance, and instructions for replacing mains fuses.

Calibration and Storage

Explains calibration procedures for the pump and needle, and guidelines for system storage.

7 Troubleshooting

Instrument-Related Problems

Addresses common issues with the instrument, including needles, flow, and system checks.

Software and Assay Problems

Guides on resolving software malfunctions and assay-related issues using provided support tools.

8 Reference Information

Technical Specifications

Lists detailed technical specifications for the Biacore X100, including general, instrument, software, and rack details.

Chemical Resistance Guide

Provides guidelines on chemical resistance for instrument components and compatibility with various solutions.

Health and Safety Declaration Forms

Includes forms for on-site service and product return, detailing safety and decontamination requirements.

Appendix A: Software Tool Texts

Software Tool Instructions

Informs users about the availability of on-screen instructions for maintenance, test, and service tools in English and local languages.

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