GE Biacore X100 - Instrument-Related Problems

7.1 Instrument related problems
General considerations
ActionProblem
Clean the flow system with Desorb
more often than once a week.
Proteins and other substances adsorb to the
walls of the flow system, giving carry-over
problems between assays.
Run Tools:More Tools:Superclean.Carry-over problems persist after Desorb
and Sanitize.
Clean the needle and wash station.Salt residues or other deposits have accumu-
lated on the needle or needle wash station.
Replace the needle (Tools:More
Tools:Replace Needle).
The needle is obstructed or damaged.
Run Tools:More Tools:Flow system
wash.
Sample consumption is unexpectedly low,
sensorgrams are irregular.
Replace the peristaltic pump tubeThe System Check and Pump Calibration
shows incorrect volume delivery from the
peristaltic pump.
(Tools:More Tools:Replace Peristaltic
Pump Tube).
Replace the pump syringe
(Tools:More Tools:Replace Syringe).
The syringe leaks or salt residues accumulate
below the syringe piston.
Damaged needle
If the needle is damaged, check the following:
ActionPossible cause
Ensure that only caps supplied by GE are used
to cap the 1.5 mL sample and reagent vials.
Never cap the water vial.
Unsuitable caps have been used
on the vials.
Change the needle as described below.
Ensure that only recommended vials are used,
see Section 8.1 Specifications, on page 84.
Unsuitable vials have been used.
Change the needle as described below.
78 Biacore X100 Operating Instructions 28961142 AD
7 Troubleshooting
7.1 Instrument related problems