5.6 Preparing and loading samples and reagents
Samples and reagents
Different reagents are used depending on the type of run to be performed. The require-
ments for your assay are displayed in the rack position list in the Control Software when
the assay is set up (see example illustration below). The list can be printed. The volumes
specified in the rack position list are minimum volumes with due consideration for dead
volumes. If dilution of samples is needed, dilute in degassed running buffer.
Follow the steps below to prepare samples and reagents.
ActionStep
Prepare the samples and reagents in 1.5 mL conical vials (available from
GE) according to the volumes and concentrations in the rack position list.
Make sure there are no visible air bubbles in the tubes. Cap the vials to avoid
evaporation during the run. Use only caps supplied for this purpose by GE.
1
Fill the 4 mL vial with distilled water. Always use fresh water filtered through
a 0.22 µm filter to remove particles. Do not cap the 4 mL vial! The water is
used for cleaning the needle during the run and during standby.
2
Load the rack as described below.3
Load the rack
Follow the steps below to load the rack in the instrument.
ActionStep
Click Load Samples in the Rack Positions dialog. You can also load the rack
using the Load Samples icon in the toolbar if you do not have an application
wizard open.
1
Wait until the Rack locked indicator below the rack compartment is switched
off.
2
Biacore X100 Operating Instructions 28961142 AD 57
5 Operation
5.6 Preparing and loading samples and reagents
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