6.7 Storage
Standby
Leave the system in standby mode if it is to be used within the next 7 days.
Biacore X100 is automatically placed in standby mode after the end of each run. In
standby mode the instrument maintains a continuous low flow of buffer or water from
the left hand bottle through the flow system to prevent accumulation of buffer residues
and to preserve the ligand on the chip. Standby can run uninterrupted for up to 7 days.
If you intend to leave the instrument for up to 7 days, check that you have:
•
a chip docked in the instrument
•
at least 120 mL buffer or water in the buffer bottle with buffer tubes inserted
•
a full water vial in the rack (the water is used for regular washes of the needle to
minimize salt deposits)
•
an empty waste bottle
You may restart the standby again after 7 days, after checking the list above.
We recommend that you use distilled and filtered water instead of buffer to
minimize salt deposits. However, if an immobilized sensor chip is docked and
will be used later, buffer may be necessary to preserve the sensor surface
during standby.
Note:
During the standby period you can leave the same buffer in the instrument
for up to 7 days. However, if you start a new run, change to a buffer fresh for
the day.
Note:
Starting standby manually
To start standby manually, select Tools:Standby in the Control Software. If you want to
close the software but leave the instrument in standby, select File:Exit, choose Standby
in the dialog that is displayed, and click Next.
Shutdown
If the instrument is to be left unused for more than 7 days or if the instrument will be
moved, perform a shutdown.
It is recommended to run Desorb and sanitize and also Clean needle and needle wash
station before starting the shutdown procedure.
The shutdown procedure flushes the flow system first with distilled water, then with 70%
ethanol and finally with air to dry the system.
74 Biacore X100 Operating Instructions 28961142 AD
6 Maintenance
6.7 Storage