GE Biacore X100 User Manual

5.5 Setting the analysis temperature
The SPR signal is sensitive to changes in temperature. It is important that a constant
temperature is maintained at the sensor chip surface throughout the run. The detection
area housing the sensor chip is maintained at a precisely controlled temperature.
The analysis temperature is fixed at 25°C in the standard instrument. With the optional
Biacore X100 Plus Package, the analysis temperature can be set to 4°C to 40°C (maximum
10°C below ambient temperature).
The analysis temperature is displayed in the status bar at the bottom of the screen.
Unstable temperature is indicated by
•
a flashing Temperature indicator on the instrument front panel,
•
a flashing temperature in the Control Software status bar and
•
a note in the event log in the Control Software during a run.
56 Biacore X100 Operating Instructions 28961142 AD
5 Operation
5.5 Setting the analysis temperature

GE Biacore X100 Specifications

General

Type	Surface Plasmon Resonance (SPR) System
Application	Biomolecular interaction analysis
Detection Method	Surface Plasmon Resonance (SPR)
Temperature Control	4-40°C
Software	Biacore X100 Control Software
Power Requirements	100-240 V, 50/60 Hz
Sample Type	Proteins, nucleic acids, small molecules
Flow Rate Range	1-100 μL/min
Data Analysis	Affinity, kinetics, concentration, specificity
Flow System	Microfluidic
Dimensions	60 cm (W) x 50 cm (D) x 45 cm (H)
Throughput	High throughput
Sample Capacity	Up to 96 samples