5.3 Preparing, loading and changing buffers
NOTICE
Always use fresh water. Replace water before each run, or at least
every 48 hours. Do not run the system without water.
Prepare buffer
Select buffer type depending on your specific experiments. For many protein interactions
you may use HBS-EP+ buffer supplied by GE.
Use degassed deionized water when preparing buffers to prevent problems with air
bubbles during the run. If the instrument is equipped with the degasser provided with
Biacore X100 Plus Package, you do not need to degas the water.
Always use buffer, fresh for the day and filtered through a 0.22 µm filter to remove par-
ticles. A volume of 200 mL is suitable for use during 24 hours.
Load buffer
Fill a clean buffer bottle with buffer and place it on the left-hand tray. Insert the two
buffer tubes. The flow system is filled with buffer automatically when a chip is docked
(see Section 5.4 Inserting a sensor chip, on page 54).
If the system is in standby, follow the instructions in Start the instrument from standby,
on page 52.
Change buffer
If you need to change buffer when the instrument is in standby, select Tools:Stop
Standby to stop the standby and change the buffer bottle. When both buffer tubes are
inserted in the new buffer, select Tools:Prime to fill the system with the new buffer.
Biacore X100 Operating Instructions 28961142 AD 53
5 Operation
5.3 Preparing, loading and changing buffers
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