Use Superclean if the regular cleaning procedures are not sufficient.
The following solutions are required for the Superclean procedure:
•
Deionized water (filtered 0.22 µm) at 40°C to 50°C
•
1% acetic acid
•
0.2 M sodium bicarbonate
•
6 M guanidine-HCl
•
10 mM HCl
If low molecular weight analytes have been used, use 50% DMSO and 10%
DMSO instead of guanidine-HCl and HCl respectively.
Note:
Normalizing the detector
This procedure adjusts the detector response to compensate for slight differences in
individual sensor chips. For best performance, run this procedure once for each new
chip. The procedure can either be run before immobilization or before the first run using
the immobilized chip. Normalization injects BIAnormalizing solution (70% glycerol in
water) over the chip surface.
Required solutions: BIAnormalizing solution
At the start of a run
Check the Normalize detector option in the System Preparations workspace, which
appears before the start of each run.
At any other time
Run the maintenance tool Normalize to normalize the detector response for all flow
cells.
NOTICE
Run Normalize with the sensor chip that will be used for the run.
Do not use Sensor Chip Maintenance for this purpose.
Biacore X100 Operating Instructions 28961142 AD 69
6 Maintenance
6.3 Cleaning
To Next Page
Loading...
