illumina NextSeq 500 - Page 56

imaging, 2-channel sequencing 40
indexing considerations 41
instrument
configuration settings 35
power button 4
start up 8
instrument maintenance
consumables 10
instrument name, customizing 9
instrument wash 24
intensities 40
InterOp files 29, 42
L
laboratory-grade water guidelines 10
lane numbering 43
lane pairs 43
locs files 42
M
maintenance, preventive 24
manage instrument
customization 9
shut down 27
metrics
base calling 40
cluster density cycles 22
intensity cycles 22
O
online training 1
output files 42
output files, sequencing 42
P
passing filter (PF) 41
phasing, prephasing 40
Phred algorithm 41
post-run wash 23
power button 4, 8
power switch 8
pre-run check 21
pre-run check errors 30
preventive maintenance 24
primer rehybridization 31
purge consumables 10
Q
Q-scores 41
quality tables 41
R
RAID error message 35
read length 12-13
reagent cartridge
overview 6
preparation 13
reservoir #28 25
reagent compartment 2
reagents
proper disposal 18
Real-Time Analysis software 1, 3
results 42
rehybridization, Read 1 31
RTA v2
termination 38
RTAv2
overview 38
run copy service 22
run duration 12
run metrics 21
run parameters
BaseSpace mode 19
edit parameters 19
standalone mode 20
run setup, advanced option 9
RunInfo.xml 29, 42
S
sequencing
user-supplied consumables 10
Sequencing Analysis Viewer 12
sequencing workflow 39
shutting down the instrument 27
sodium hypochlorite, wash 25
software
automatic update 27
configuration settings 35
image analysis, base calling 3
initialization 8
instrument setup 9
manual update 27
on-instrument 3
run duration 12
Document # 15046563 v04
For Research Use Only. Not for use in diagnostic procedures.
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NextSeq 500 System Guide