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Figure 1.18 - OpenSPR™ instrument setup including optical, fluidic and Sensor Chip components
The Sensor Chip consists of a nanogold coating (visible
as a red spot) on a glass substrate [Figure 1.19].
Without a Sensor Chip, the OpenSPR™ system cannot
be used. For typical SPR biosensing experiments, the
surfaces must be given specific functionality by
immobilizing one of the binding partners (the ligand)
onto the surface. There are a number of ways to do
this, and Nicoya Lifescience Inc provides a variety of
pre-prepared surface chemistries to ensure consistent
and high quality ligand immobilization. See OpenSPR
Kinetics Handbook for more details on the Sensor
Chips.
The Fluidics Block is designed to continuously pump running buffer from the inlet tubing through
a microchannel in the Flow Cell and over the sensor surface. The Sensor Chip is aligned and
secured against the Flow Cell and Fluidics Block using a magnetic sample holder [Figure 1.20].
Sample analyte is loaded into the system and introduced to the sensor surface using the XT
autosampler.
Figure 1.19 - Sensor Chip with nanogold (red
spot)
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