Nikon A1 User Manual

Chapter 3. Operation of the Microscope A1
11
3.6 Perfect Focus System Operation (with the Front Operation Panel)
(1) Check that the dichroic mirror IN/OUT lever is
set to IN.
(2) Set the objective to be used in the optical path
and adjust the focus with the focus knob on
the microscope to focus on the sample.
(3) Press the PFS-ON button on the front
operation panel.
(When the PFS starts, the PFS-ON indicator
lights green.)
OUT
DICHROIC MIRROR
IN
Dichroic mirror IN/OUT lever
(4) Focus on the position at which focus is maintained with the offset dial on the PFS offset controller and
start microscopy.
Once the PFS operation is started, focusing with the focus knob on the microscope is disabled.
Registering offset amount: The focus position adjusted with the offset dial can be registered with the
PFS-MEMORY button.
Recalling offset amount: Registered focus position can be called with the PFS-RECALL button.
L80
EYE
DISPLAY
MEMORY
PFS
ON RECALL
Z
-
RESETBRIGHTNESS
R100L100
FOCUS
1X
1.5X
PFS
DOWN
UP
PFS
OFFSET
CONTROLLER
FINE
COARSE
Figure Ti-E Front Operation Panel and Offset Controller
PFS-ON
button/indicator
PFS-MEMORY
button/indicator
PFS-RECALL
button/indicator
FINE/COARSE selector
button

Nikon A1 Specifications

General

Type	Confocal Microscope
Software	NIS-Elements
Laser Lines	405 nm, 488 nm, 561 nm, 640 nm
Detector	GaAsP PMT detectors
Imaging Modes	Confocal, Spectral, FRAP
Scanning Method	Galvanometer-based scanning
Objectives	Compatible with Nikon CFI objectives
Dimensions	Varies with configuration
Weight	Varies with configuration
Laser	Multiple laser lines available